Hi. I'll be receiving some library preps from a collaborator next week that were made with TruSeq Stranded mRNA kit. We will run them for her on our MiSeq.
She keeps emphasizing "STRANDED" in her messages. I just want to check here to make sure there isn't some special way of running stranded mRNA libraries on the MiSeq. I haven't run any stranded libraries before. I'm assuming the difference will come in the analyses.
Please let me know if I need to do anything special for the run. I have looked at the protocol and it doesn't say anything about special running directions.
Thanks for taking the time to answer my silly newbie-sounding question!
She keeps emphasizing "STRANDED" in her messages. I just want to check here to make sure there isn't some special way of running stranded mRNA libraries on the MiSeq. I haven't run any stranded libraries before. I'm assuming the difference will come in the analyses.
Please let me know if I need to do anything special for the run. I have looked at the protocol and it doesn't say anything about special running directions.
Thanks for taking the time to answer my silly newbie-sounding question!
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