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We are trying to troubleshoot contamination issues that we are having in our ChIP-seq experiments. It seems that recently we have been sequencing constructs that have been cloned in the lab in other experiments in our IPd samples. We are trying to decided how to proceed, how many precautions to take. We are interested in hearing what others do. Do you have a designated area, designated equipment, etc.? At what point in the procedure do you begin to use this designated area? As early as the IP or just at the amplification step? Any and all of your thoughts and suggestions would be greatly appreciated!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM