The read starts reading the index, then the target specific primers and then the insert itself, correct?
So for example, I am using a 100 cycle kit, my index is 8bp long and I am doing 50bp PE reads, how much of my target insert would actually be sequenced? 92bp?
So for example, I am using a 100 cycle kit, my index is 8bp long and I am doing 50bp PE reads, how much of my target insert would actually be sequenced? 92bp?
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