newbie question on generation of libraries..

[arkilis]

When I read bioinformatics book, I got "However, generation of libraries is a bottleneck. Most researchers are not able to do this." .Is there anyone know why? Thx

[Heisman]

What book are you reading and what year was it published? That is not an accurate statement, I don't think. The bottlenecks are usually either obtaining relevant clinical samples, obtaining sequence data if you have a small sequencing core with lots of samples to process, or properly analyzing the data if you lack computational resources/knowledge/experience in analyzing these datasets.

[arkilis]

What book are you reading and what year was it published? That is not an accurate statement, I don't think. The bottlenecks are usually either obtaining relevant clinical samples, obtaining sequence data if you have a small sequencing core with lots of samples to process, or properly analyzing the data if you lack computational resources/knowledge/experience in analyzing these datasets.

thanks for your reply. The reading materials is a guide from BioScience. page 49. Thanks for your help!

https://www.dropbox.com/s/j2hvv0qbxnh48jm/NGS%20Overview.pdf

[SNPsaurus]

arkilis, you are reading product literature from Axygen, not a book with a neutral point of view. While library-making can be slow and a stumbling point for someone new, it quickly becomes routine.

Axygen sells 96-well plates suited for robotics. You have to read product literature from a company with a certain amount of criticality (and I say this as a founder of a commercial entity myself). In this case, it helps Axygen to encourage people to think about how to make lots of libraries more easily, which maybe will give them the idea to use automation and Axygen's plates. That may be perfectly valid, but not always the solution for each person reading the guide.

[arkilis]

arkilis, you are reading product literature from Axygen, not a book with a neutral point of view. While library-making can be slow and a stumbling point for someone new, it quickly becomes routine.

Axygen sells 96-well plates suited for robotics. You have to read product literature from a company with a certain amount of criticality (and I say this as a founder of a commercial entity myself). In this case, it helps Axygen to encourage people to think about how to make lots of libraries more easily, which maybe will give them the idea to use automation and Axygen's plates. That may be perfectly valid, but not always the solution for each person reading the guide.

Thanks, so do you have any recommendation on new bioinformatics people?