We've updated the AllSeq NGS Knowledge Bank to reflect the latest Illumina instruments - all the specs in one place!
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Updated Illumina Specs Table
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Can the 2500 Rapid mode really produce 600M clusters in the two 'lanes'? I've seen results more along 150-180M clusters. Maybe I'm thinking of passing filters and this table is not, but I don't think I've seen the lanes loaded to make 300M clusters each either.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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Originally posted by SNPsaurus View PostCan the 2500 Rapid mode really produce 600M clusters in the two 'lanes'? I've seen results more along 150-180M clusters. Maybe I'm thinking of passing filters and this table is not, but I don't think I've seen the lanes loaded to make 300M clusters each either.
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Ahh, 600M X 300 bp = 180 Gb, so that would be 150M clusters per lane x 2 reads x 150 bp. I think you are counting clusters in some columns and total reads possible (2 per cluster) in others.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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Originally posted by SNPsaurus View PostCan the 2500 Rapid mode really produce 600M clusters in the two 'lanes'? I've seen results more along 150-180M clusters. Maybe I'm thinking of passing filters and this table is not, but I don't think I've seen the lanes loaded to make 300M clusters each either.
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Originally posted by SNPsaurus View PostAhh, 600M X 300 bp = 180 Gb, so that would be 150M clusters per lane x 2 reads x 150 bp. I think you are counting clusters in some columns and total reads possible (2 per cluster) in others.
For the HiSeq 2500 Rapid Mode we have:
2 flow cells X 300M reads/flow cell X 2x150bp reads
2*300M*2*150b=180Gb
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Right, sorry for the confusion. The single flow cell NextSeq got me messed up going back to the two-flow-cell HiSeq.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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Originally posted by SNPsaurus View PostRight, sorry for the confusion. The single flow cell NextSeq got me messed up going back to the two-flow-cell HiSeq.
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