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  • #16
    Illumina is going to re-introduce ribozero equivalent kits in 2020.

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    • #17
      For those seeking a bacterial rRNA depletion kit, NEB has just released a kit that works very well in our hands.

      Effective depletion of rRNA from bacteria (gram-positive and gram-negative organisms), from monocultures or samples with mixed bacterial species.

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      • #18
        Originally posted by cement_head View Post
        Has anyone tried this kit from ZYMO? Usually ZYMO stuff is pretty good...

        https://www.zymoresearch.com/product...ersal-cdna-kit
        DISCLAIMER: I work for Zymo

        We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.

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        • #19
          According to rumors, RiboZero will make a comeback. Not exactly the same products though; it will use a new chemistry.

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          • #20
            Illumina did release a new version of RiboZero+ yesterday: https://www.illumina.com/products/by...depletion.html

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            • #21
              Originally posted by jjlin View Post
              DISCLAIMER: I work for Zymo

              We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.
              Interesting. Appears to use a strand-denaturation/hybridization-based normalization to remove/reduce concentrations of highly represented cDNAs. That is, enzymatic digestion of DNA/RNA duplexes that form due to C0t kinetics.

              This would make it species agnostic. Specified to work down to 100ng or somewhat below that with extended hybridization times.

              Most of the charm of the old Truseq Ribozero-depletion library kits was that although they specified a limited range of species, eg "human/mouse/rats" the probes were very long (not oligos) and so actually seemed to work on any animal. For example they worked on moth RNA in our hands.

              --
              Phillip

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              • #22
                Thanks. No manual available yet. List price is $82 per reaction.

                Originally posted by GenoMax View Post
                Illumina did release a new version of RiboZero+ yesterday: https://www.illumina.com/products/by...depletion.html

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