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  • rate my tophat2 results :-)

    Hi,
    New in RNA-seq and without any help in my lab, i would like to ask you your advice about my mapping results. (In my opinion, it's ok, but i will be very happy if experimented poeple can confirm it or not).
    here are align summary tophat results and accepted_hits.bam analysed by samtool flagstat.

    Left reads:
    Input : 16641634
    Mapped : 15985940 (96.1% of input)
    of these: 4863655 (30.4%) have multiple alignments (7966 have >20)
    Right reads:
    Input : 16641634
    Mapped : 15922959 (95.7% of input)
    of these: 4834402 (30.4%) have multiple alignments (7972 have >20)
    95.9% overall read mapping rate.

    Aligned pairs: 15508899
    of these: 4652618 (30.0%) have multiple alignments
    87521 ( 0.6%) are discordant alignments
    92.7% concordant pair alignment rate.

    =============

    47082928 + 0 in total (QC-passed reads + QC-failed reads)
    0 + 0 duplicates
    47082928 + 0 mapped (100.00%:-nan%)
    47082928 + 0 paired in sequencing
    23593110 + 0 read1
    23489818 + 0 read2
    44621168 + 0 properly paired (94.77%:-nan%)
    45651258 + 0 with itself and mate mapped
    1431670 + 0 singletons (3.04%:-nan%)
    2790766 + 0 with mate mapped to a different chr
    86220 + 0 with mate mapped to a different chr (mapQ>=5)

    Thanks for your advice.

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