i am planning to do some exome sequencing and now I am working on standardizing the covaris protocol to get a gDNA complex size range around 225 basepairs.
I get a good peak at 225 basepairs and a lot of DNA within the region i want but there is some amount of DNA outside the range which are either too small or too big. Should I just ignore it or do I need to do a size selection by running a gel. I am skeptical to do that because of the yield issues during gel purification.
Broad uses covaris and does not do size selection by gel purification and they are very confident because they get a very accurate peak. I am not sure if my peak is that accurate. I am attaching the results of my bioanalyzer run. Can some one help me with this and check if its a nice peak to go ahead with it?
Thanks
I get a good peak at 225 basepairs and a lot of DNA within the region i want but there is some amount of DNA outside the range which are either too small or too big. Should I just ignore it or do I need to do a size selection by running a gel. I am skeptical to do that because of the yield issues during gel purification.
Broad uses covaris and does not do size selection by gel purification and they are very confident because they get a very accurate peak. I am not sure if my peak is that accurate. I am attaching the results of my bioanalyzer run. Can some one help me with this and check if its a nice peak to go ahead with it?
Thanks
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