Different read lengths in same paired end run

WinterIsComing · 01-07-2019, 04:06 PM

Hi all, Can Miseq be programmed to read different lengths for read 1 and read 2?

Also, if one skips the cycles used for indexing can those cycles be added onto the reads? For example, instead of a 2 X 75 run with 2 index reads of 8 bases, could one just do a 2 X 83 run with no index reads?

torben · 01-08-2019, 01:23 AM

Yes, and yes. For the total number of cycles you can run see http://emea.support.illumina.com/bul...in-my-kit.html

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01-07-2019, 04:06 PM	#1
WinterIsComing Junior Member Location: USA Join Date: Jan 2019 Posts: 3	Different read lengths in same paired end run Hi all, Can Miseq be programmed to read different lengths for read 1 and read 2? Also, if one skips the cycles used for indexing can those cycles be added onto the reads? For example, instead of a 2 X 75 run with 2 index reads of 8 bases, could one just do a 2 X 83 run with no index reads?

01-08-2019, 01:23 AM	#2
torben Member Location: Norway Join Date: Oct 2012 Posts: 20	Yes, and yes. For the total number of cycles you can run see http://emea.support.illumina.com/bul...in-my-kit.html