Roche 454 RL adapters.. help!!!

Vartul · 01-26-2012, 08:48 AM

Hi,

We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul

RCJK · 01-26-2012, 02:57 PM

This technical bulletin should help answer your questions.

Vartul · 01-27-2012, 02:39 AM

Dear RCJK, many thanks for the technical bulletin.

kevin zhang · 09-11-2020, 08:23 AM

Quote:

Originally Posted by Vartul

Dear RCJK, many thanks for the technical bulletin.

Dear sir, can you give me this technical bulletin?thank you so much.

ellli555 · 01-14-2021, 12:06 AM

Hi!
could you upload this technical bulletin again?
I cannot download it from the link you provided already a decade ago...
Thanks!
Ellli

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01-26-2012, 08:48 AM	#1
Vartul Junior Member Location: Glasgow Join Date: Dec 2011 Posts: 3	Roche 454 RL adapters.. help!!! Hi, We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction? Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction. Thank you, Vartul

01-27-2012, 02:39 AM	#3
Vartul Junior Member Location: Glasgow Join Date: Dec 2011 Posts: 3	Dear RCJK, many thanks for the technical bulletin.

01-14-2021, 12:06 AM	#5
ellli555 Junior Member Location: greece Join Date: Jan 2021 Posts: 2	Hi! could you upload this technical bulletin again? I cannot download it from the link you provided already a decade ago... Thanks! Ellli