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Old 07-02-2012, 10:02 AM   #1
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Location: Boston

Join Date: Jun 2012
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Default Amplification of 500pg of RNA for illumina sequencing libraries

We need to amplify very small amounts of RNa ~500pg and generate libraries for the Illumina HiSeq. I have looked at a variety of kits etc. Does anyone have a method that is working well with minimal 3'-bias?

Many kits seem to mix oligo-DT and random primers to try to get rid of 3'-bias. Does anyone have a good explanation for this? Why not just random prime?

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