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Old 04-03-2015, 06:54 AM   #1
arum
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Location: Paris

Join Date: Apr 2015
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Default TruSeq Stranded total RNA - Cooling block passive for synthesizing 1st strand cDNA

Dear all,

I start my 1st automated experiment with TruSeq stranded total RNA. Unfortunately, my pipetting platform doesn't have a cooling block (I didn't choose this option when I bought it), my question is :

- For the "Synthesize 1st strand cDNA" and "Adenylate 3' Ends" steps, basically, we need to incubate samples at 70C then hold at 4C. Can I process directly the next step without this 4C, or use a cooling block passive (not controled temperature, so the temperature can be 4, 6, 10C...)?

Does anyone faced this problem? Help me please.

Many thanks,
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