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  • Miseq illumina primer

    Hi,

    I am new here and also in sequencing area. I am not sure if I can ask such a basic question here or not. Actually, I'd like to sequence my 16S rRNA data for 3 different regions (v1-v3, v3-v5 and v6-v9). I have to design three different primer for sequencing with Miseq illumina system. I have read (Cporaso 2010 and 2012) papers but I have still problem with designing primers:

    1- I don't know where I can find the Miseq illumina adaptor.
    2- If it is some sequence constant for all regions or should be different.
    3- How can I design the pad part.
    4- The linker part.
    5- The barcode
    6- And also 12-base error-correctong Golay.

    Could anyone explain them for me or any basic and advanced literatures I can learn from?!

    Thanks,
    Rozita

  • #2
    Here is an application note from Illumina that covers 16S sequencing on MiSeq: http://www.illumina.com/documents/pr..._miseq_16S.pdf

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