Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Hisat2 output is empty

    I run this command:

    hisat2 -p 40 -q -x Ene_index --sra-acc SRR13696274 --al alignedRNA_1 -S alignment1

    and the output says:
    4249510 reads; of these:
    4249510 (100.00%) were unpaired; of these:
    3194713 (75.18%) aligned 0 times
    1045690 (24.61%) aligned exactly 1 time
    9107 (0.21%) aligned >1 times
    24.82% overall alignment rate

    However, the produced alignedRNA_1 file is empty

    Does anyone know what the problem may be?

    Thanks

  • #2
    Ok - here's the reply I got from the developers, which solved the problem

    Hi Niloofar,

    Thank you for your reporting.
    Could you download the SRA file (use fastq-dump program from SRAToolkit) and run HISAT2 with the file again?
    There is a problem when you use --sra-acc and --al (--al*, --al-conc* , --un-conc* options) together, HISAT2 doesn't create read files properly. We'll fix it.

    Thanks
    Chanhee

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 08:47 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    59 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    54 views
    0 likes
    Last Post seqadmin  
    Working...
    X