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Hi all,
Does anyone have a sample of a fastq file generated by the HiSeq 2000 sequencer (with only a few reads) ?
Our team will be analyzing HiSeq 2000 data soon but we only have GAIIx data in our hands at the moment.
Concerning GAIIx sequencers, we know that a lane is made of 120 tiles organized in a 60*2 fashion. These tiles represent locations where the machine take pictures. Knowing how tiles are organized in the lane allows one to analyze the spatial distribution of the clusters within the lane, biaises, any problems, etc...In the fastq files generated by GAIIx, the identifier of the read contains information on the lane, the tile and coords within the tile. Is there something equivalent for Hiseq sequencers ? I was not able to link on the web HiSeq 2000 sequencer and the concept of tile (maybe because the optic system changed).
Does someone have any clue on this point ?
Is it possible to physically map reads on the lane for Hiseq similarly to what we can do for GAIIx sequencers ?
regards,
tony
Does anyone have a sample of a fastq file generated by the HiSeq 2000 sequencer (with only a few reads) ?
Our team will be analyzing HiSeq 2000 data soon but we only have GAIIx data in our hands at the moment.
Concerning GAIIx sequencers, we know that a lane is made of 120 tiles organized in a 60*2 fashion. These tiles represent locations where the machine take pictures. Knowing how tiles are organized in the lane allows one to analyze the spatial distribution of the clusters within the lane, biaises, any problems, etc...In the fastq files generated by GAIIx, the identifier of the read contains information on the lane, the tile and coords within the tile. Is there something equivalent for Hiseq sequencers ? I was not able to link on the web HiSeq 2000 sequencer and the concept of tile (maybe because the optic system changed).
Does someone have any clue on this point ?
Is it possible to physically map reads on the lane for Hiseq similarly to what we can do for GAIIx sequencers ?
regards,
tony