Hi,
I am relatively new to the Genomics/Transcriptomics game and about to prep Illumina TrueSeq Total RNA libraries from fungal RNA. I read through the instructions several times, everything seems quite clear to me except the Normalize/Pooling step at the very end.
Isn't the normalization step just important when doing comparative Transcriptomics?
I need transcriptomes just to assist genome annotation, so normalization is not a big issue. Am I right?
Thank you for your help!
I am relatively new to the Genomics/Transcriptomics game and about to prep Illumina TrueSeq Total RNA libraries from fungal RNA. I read through the instructions several times, everything seems quite clear to me except the Normalize/Pooling step at the very end.
Isn't the normalization step just important when doing comparative Transcriptomics?
I need transcriptomes just to assist genome annotation, so normalization is not a big issue. Am I right?
Thank you for your help!
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