Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • cummeRbund JS Distances (Jensen–Shannon divergence)

    Has anyone used the cummerRbund bioconductor package on RNA-seq data?

    How does one interpret the data?

    I have 6 samples no replicates, of 2 different tissues. cummeRbund shows clear clustering of the samples tissue-specifically on a JS Distance scale.

    The scale in my situation runs from 0 to 0.2.

    So tissue 1 lies at 0,1
    tissue 2 lies at 1,8

    This is run on CuffDiff for a tissue-specific set of genes to tissue 1 known to be expressed from RTPCR and so on.

    Then how do I interpret this? Clustering is clear, but why not 0.05 and 0.9 for the two groups? or something that tells that tissue 1 samples are 'close together'?

    I hope this is not too trivial a question, and any comment is appreciated.

  • #2
    What is the difference, and what is the similarity. Can Javascript writer can write actionscipt ? and Can Actionscipt writer can work in javascript? Please Help. Confused by myself in both of them. Which would be better to learn.
    ________________________
    healthy recipes ~ australia lifestyle ~ life in australia
    Last edited by andrikaa; 02-28-2014, 02:41 AM.
    welcome to your world

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 08:47 AM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    54 views
    0 likes
    Last Post seqadmin  
    Working...
    X