Hello Fellow NGS users,
I recently sequenced my micro biome (Five body sites) using the Illumina 16SrRNA V4 metagenomics platform. The paired reads were analyzed on base space with sample information showing 100% quality filtering. From the classification statistics, one of the samples had over 50% as unclassified at Kingdom level, phylum, class, order, family, genus and species levels. What could be responsible for this and is there a way of extracting the sequence reads that were reported as unclassified.
I recently sequenced my micro biome (Five body sites) using the Illumina 16SrRNA V4 metagenomics platform. The paired reads were analyzed on base space with sample information showing 100% quality filtering. From the classification statistics, one of the samples had over 50% as unclassified at Kingdom level, phylum, class, order, family, genus and species levels. What could be responsible for this and is there a way of extracting the sequence reads that were reported as unclassified.
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