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Old 03-26-2015, 04:25 AM   #1
yueli
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Location: china

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Default Blast2go

Hi,

I only have ESTs sequencing by hand and want to make annotation to these sequencing.

Can I use Blast2go to annotate these sequencing?

Thanks!

Li




gi=84601702
gi=84603312
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gi=84611454
gi=84612361
gi=84613165
gi=84613222
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Old 03-30-2015, 03:47 AM   #2
sgoetz
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Yes, just make sure you have your EST sequences in Fasta format. Load them into Blast2GO and proceed with the steps: NCBI Blast, GO-Mapping, B2G Annotation.

You can also add InterProScan domain-based GO terms to you sequences. Run InterPro and then run "Merge InterProScan GOs to annotations".

To use Blast2GO you have to register for a free basic account: https://www.blast2go.com/blast2go-pr...register-basic
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Old 04-14-2015, 11:25 PM   #3
yueli
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Thanks, sgoerz.
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