Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
DESeq2: diff gene expression between species using gene-specific normalization factor mra Bioinformatics 4 12-01-2014 06:17 AM
DESeq2 finding differential expression changes with libraries of different sizes mistrm RNA Sequencing 7 08-20-2014 08:01 AM
what is a read group? wenhuang Bioinformatics 4 04-03-2014 05:46 AM
Differential gene expression across life cycle with DESeq2 DrWorm Bioinformatics 7 03-03-2014 12:24 PM
Read Group comorado Bioinformatics 3 05-06-2010 08:36 AM

Thread Tools
Old 04-28-2015, 01:19 AM   #1
Junior Member
Location: Goettingen, Germany

Join Date: Aug 2010
Posts: 9
Default DESeq2 - Log2fc when all expression is 0 in one group?

Hi all,

So I've got an experiment where for certain experimental reasons, there is absolutely no expression in one group of some genes and robust expression in the other group. Yet DESeq2 reports a log2fc score - how is that calculated? Is there some kind of pseudocount added to a term?
bryand is offline   Reply With Quote
Old 04-28-2015, 07:24 AM   #2
Michael Love
Senior Member
Location: Boston

Join Date: Jul 2013
Posts: 333


here's an answer i posted on the bioc support site to the same question
Michael Love is offline   Reply With Quote

deseq2, rna-seq advice

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:19 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO