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Old 06-15-2017, 01:59 PM   #1
Postdoc Cancer Bioinformatics
Location: Olso, Norway

Join Date: Dec 2014
Posts: 17
Default is this a technical replicate or biological replicate

i know that biological replicate is one taken from different organism/sample while technical replicate is one from same organism/sample.

However, I am not able to understand that a cell line treated with a drug three times separately to generate RNA-Seq data will be considered as biological replicate or technical replicate or a biological replicate but not in a strict sense. Also, will this be applicable for control sample?

Thanks in advance
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Old 06-15-2017, 02:58 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
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I think it would be biological replicates and the same goes for control which should be treated with solvent without the drug. I imagine that there will be other treatments such as exposure time and dose as well in such experiments.
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Old 11-28-2017, 07:19 AM   #3
Location: NL

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As the variation during cultering is expected (and often is) far more variable then the technical reproducibility the biological replicate would be to repeat the actual incubations i.e. three times (for a triplicate).
A technical replicate would be having the same RNA sample sequenced two times (i.e. a duplo).
Often an experiment is repeated three times, getting n=3 for the experiment and statistics. In the experiment an incubation can be in duplo (i.e. two flasks stimulated with the same compound). Each incubation is then sequenced once.
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cell line, replicates, rna-seq, rna-seq advice, rna-seq design

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