Hello all,
Does DESeq use replicate info in estimateSizeFactors?
For RNA seq without replicates - will it be OK to normalize the data using:
estimateSizeFactors
(and then dividing each column by the size factors?)
Also if I have several conditions, and have a replicate only for one of them - is it OK to use estimateSizeFactors?
Thanks.
Does DESeq use replicate info in estimateSizeFactors?
For RNA seq without replicates - will it be OK to normalize the data using:
estimateSizeFactors
(and then dividing each column by the size factors?)
Also if I have several conditions, and have a replicate only for one of them - is it OK to use estimateSizeFactors?
Thanks.
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