Hi,
I want to try G&T-seq protocol for sequencing RNA and DNA from one single cell. I have already experience in using Smart-seq 2 and MDA protocols.
But I'm interested to know if someone has tried this protocol and if it's easy to set up, especially the first part of the method to capture mRNA using magnetic beads.
In general what are the limitations and difficulties in this protocol?
Tnx
F
I want to try G&T-seq protocol for sequencing RNA and DNA from one single cell. I have already experience in using Smart-seq 2 and MDA protocols.
But I'm interested to know if someone has tried this protocol and if it's easy to set up, especially the first part of the method to capture mRNA using magnetic beads.
In general what are the limitations and difficulties in this protocol?
Tnx
F
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