Hi!
I want to look for changes in mRNA "before" and "after" an intervention. I only use one patient (two time point) for this test.
I have two .BAM files from Tophat2.
When I use cuffdiff, how can I mask all abundant sequences in hg19? I have hg19 from iGenomes and in the "abundant sequences" there are alot of FASTA files.
Pls help, thank you!
I want to look for changes in mRNA "before" and "after" an intervention. I only use one patient (two time point) for this test.
I have two .BAM files from Tophat2.
When I use cuffdiff, how can I mask all abundant sequences in hg19? I have hg19 from iGenomes and in the "abundant sequences" there are alot of FASTA files.
Pls help, thank you!
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