Hi,
I'm planning a de novo sequencing on isolated gram positive bacteria.
I heard from the sequencing provider that this technique is really sensitive to contamination. Especially for gram positive bacteria, what I understand is the residue or sugar from cell wall easily cause problem in the library preparation.
The isolate is gram positive cellulose degrading bacteria, the situation might worse.
So I plan to grow it on cellobiose, but meanwhile, if there is a good way to remove polysaccharide after the DNA extraction?
Thanks in advance!
I'm planning a de novo sequencing on isolated gram positive bacteria.
I heard from the sequencing provider that this technique is really sensitive to contamination. Especially for gram positive bacteria, what I understand is the residue or sugar from cell wall easily cause problem in the library preparation.
The isolate is gram positive cellulose degrading bacteria, the situation might worse.
So I plan to grow it on cellobiose, but meanwhile, if there is a good way to remove polysaccharide after the DNA extraction?
Thanks in advance!
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