I have an experiment planned in which RNA samples will be collected during a clinical study over the course of a year. I plan to use Qiagen RNeasy Micro kit for RNA prep
Which is the best option to minimize biases and maximize RNA stability and integrity (for future use in RNASeq):
1) Save cells as pellet in -80C; prepare using RNAprep kit after all samples are collected (roughly 1 year storage time)
2) Start RNAPrep procedure, adding the first buffer and homogenizingg; saving homogenized cell lysate for up to 3 months in -80C; and processing collected samples every ~3 months.
3) Prepare each RNA sample immediately and store as RNA in -80C until ready to perform library prep on all clinical samples simultaneously.
Happy for advice on best method and rationale!
Thanks
Noa
Which is the best option to minimize biases and maximize RNA stability and integrity (for future use in RNASeq):
1) Save cells as pellet in -80C; prepare using RNAprep kit after all samples are collected (roughly 1 year storage time)
2) Start RNAPrep procedure, adding the first buffer and homogenizingg; saving homogenized cell lysate for up to 3 months in -80C; and processing collected samples every ~3 months.
3) Prepare each RNA sample immediately and store as RNA in -80C until ready to perform library prep on all clinical samples simultaneously.
Happy for advice on best method and rationale!
Thanks
Noa
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