Go Back   SEQanswers > Sequencing Technologies/Companies > 454 Pyrosequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
454 Sequencing Assembly/MIDs clostridium40 454 Pyrosequencing 7 09-30-2012 09:53 PM
Sorting and removing MIDs from fastq file (Roche 454) ewilbanks Bioinformatics 25 01-10-2012 04:29 AM
amplicons and MIDs for GS junior Romilio Espejo Sample Prep / Library Generation 0 08-09-2011 12:19 PM
454 MIDs aligenie 454 Pyrosequencing 18 04-22-2011 11:07 PM
amplicon with mids 454 litali Bioinformatics 1 07-27-2010 07:19 AM

Thread Tools
Old 02-21-2012, 12:17 PM   #1
Junior Member
Location: pnw

Join Date: Feb 2012
Posts: 6
Default 454 Junior MIDs

Hey folks.

I'm attempting to do a 454 Junior run with 54 pooled libraries, and it seems that there are only 14 suggested MIDs for use with the GS Junior chemistry. Supposedly these are optimised for easy differentiation, but theoretically you can create your own additional tags as well. Does anybody have success with homebrewed MID tags and care to share some sequences? Or is there a way to combine multiple tags? Some of my amplicons could be slightly larger than the available read length, so putting an MID on each end might not work.

Any feedback is appreciated!
newendophytologist is offline   Reply With Quote
Old 02-21-2012, 02:15 PM   #2
Junior Member
Location: Emory University - Decatur, GA

Join Date: Dec 2011
Posts: 2
Default Extended MID List

Here is the extended List of MIDs I reference when designing barcoded adaptors that are incorporated during PCR. I have only used the first 18 on this list and don't know how well any of the others work.

These are not the MIDs for the RAPID ligation-library prep. Those are available in another Tech Bulletin and on IDTs website. I can dig up that list if required.
Attached Files
File Type: pdf TB-0905_Technical_Bulletin_Roche_MID_Adaptors.pdf (489.0 KB, 81 views)
sdl11 is offline   Reply With Quote
Old 02-24-2012, 12:34 PM   #3
Nicole 454 Sequencing
Location: Branford

Join Date: Apr 2011
Posts: 19

Hi Newendophytologist,

As sdl11 pointed out, there is an extended list of MIDs with an additional 120 MID adapters. These are in addition to the 12 MIDs provided in the GS Rapid Library MID Adapters Kit. More information on these Rapid Library MID Adapters is found in Technical Bulletin No. 2010-010 on our customer accessible website

Technical Support Scientist
454 Life Sciences, A Roche Company
Nicole 454 Sequencing is offline   Reply With Quote

454, junior, mid, multiplex

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 05:22 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO