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Old 04-10-2012, 01:21 PM   #1
eab
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Location: Maryland

Join Date: May 2011
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Default NEB adaptors for barcoded paired-end mRNA-seq

Has anyone tried the NEB oligos for barcoded, paired-end mRNA seq as a replacement for the TruSeq adapters in the Illumina kits? I am wondering if the hairpin adapter and the addition of barcodes in PCR rather than adapter ligation causes any change in sequence data obtained.
Thanks for any and all input,
eab
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