Hi All,
I'm working on an RNA sequencing protocol starting from lymph nodes stored in RNAlater but my yields of total RNA have been disappointing–3-5ug from <50mg desiccated tissue. Does anyone have experience with RNAlater samples? What kind of yields should I expect?
I'm using the TissueRuptor with RNAzol RT (MRCgene) for precipitation of total RNA.
thanks,
Will
I'm working on an RNA sequencing protocol starting from lymph nodes stored in RNAlater but my yields of total RNA have been disappointing–3-5ug from <50mg desiccated tissue. Does anyone have experience with RNAlater samples? What kind of yields should I expect?
I'm using the TissueRuptor with RNAzol RT (MRCgene) for precipitation of total RNA.
thanks,
Will