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Old 07-18-2012, 12:26 AM   #1
Junior Member
Location: Tokyo

Join Date: Jul 2012
Posts: 3
Default 454 junior user - hello everyone

Hi everyone,
I am Encarnita, french-spanish immunologist working in Japan.
I am using 454 Lifescience Tech. since more than 3 years, but autonomously using 454 Junior since a year. I've been getting many good infos by surfing on that great forum , but I registered only recently !
I've been suffering several troubles since I perform the 454 libraries preparation, emPCR and sequencing by my self, such as too few enriched beads, too much enriched, never-ending washing steps, few passed filter reads out of many raw reads, software upgrade issues leading to new setup after 12 successfull run....
I have no experience on other platforms, but I am eager to learn and I really want to understand precisely what's going on with the 454 steps.
I'll be pleased to help also, if I have any relevant info.
All the best to seqanswers community !
Encarnita is offline   Reply With Quote

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