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Old 10-10-2013, 06:46 AM   #1
CCBIO
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Default FFPE sequencing on 454 GS Junior

Hello everyone! Does someone have experience in sequencing amplicon libraries obtained from FFPE? I've heard that there are some specific Taq polimerase that can be used when amplifying FFPE (for example RESTORASE). I was wondering if these Taq are reliable and high-fidelity and if they can be used to prepare amplicon libraries to be sequenced on a 454 GS Junior platform. Does Anyone want to share some experience? Thanks
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