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Old 06-18-2014, 02:44 AM   #1
andrewsssa
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Location: Qatar

Join Date: Mar 2014
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Default Generate frame-shift data from BAM files

I am analyzing paired-end sequences I obtained from phage display where the inserted fragment is cDNA. Since there are three frames and the chunk of cDNA was inserted randomly, most of the sequences I expect to be out of frame. So, I need to get the frame data.

I have run TopHat to get the BAM files, which nicely shows chromosomal alignments. But does anyone have ideas about how to convert chromosomal position to nucleotide positions or protein sequence?
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