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Old 05-08-2015, 02:44 PM   #1
SDPA_Pet
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Location: US

Join Date: Apr 2013
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Default Purification Kits

Hi guys,

I am doing illmumina amplicon sequencing and metagenomics sequencing from environmental samples. We just purified the a plate. I measured the DNA concentration before and after purification. I found there is only 2% re-coverage. Total DNA concentration less than 1ng/ul after purification. This is horrible.

What we used is a gel purification kit and so inefficient (slow).

what DNA purification kits do you normally use for the illumina sequencing? for Nextera kits? We just start to do Illumina sequencing. Back to old days, we did 454 sequencing and it never happened like this.

Any efficient and better kits recommend?
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Old 05-13-2015, 06:10 AM   #2
Antonvh
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Hi!

I would definetly give SPRI methods a try. The Nextera protocol says to use Zymo columns, but we have tried magnetic beads and it works just fine, and the recovery is really high (90+%).
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