Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Miseq multiplexed sequencing with NEB library prep kits nvd Introductions 4 08-25-2017 12:28 PM
Alternatives to Cycle Sequencing Kits Amalynne Sanger/Dye Terminator 0 12-11-2014 05:20 AM
Sequencing bacterial mRNA with Illumina stranded kits Noa RNA Sequencing 1 11-14-2012 03:13 AM
Kits for sequencing long amplicons on MiSeq odile Illumina/Solexa 0 03-07-2012 12:50 PM

Thread Tools
Old 09-27-2017, 04:03 AM   #1
Registered Vendor
Location: Vienna, Austria and NH, USA

Join Date: Aug 2012
Posts: 49
Lightbulb Release of SLAMseq kits for metabolic RNA sequencing

Today Lexogen, the transcriptomics and next-generation sequencing company, launched the SLAMseq product family for high throughput metabolic sequencing of RNA. The kits are based on a new technology developed at the Institute of Molecular Biotechnology (IMBA) at the Vienna BioCenter in Austria, which was published in Nature Methods.

Current RNA-Seq methods can measure steady-state RNA levels. However, they cannot resolve the underlying kinetics of RNA turnover that contribute to total RNA levels. This has been a limitation for the application of RNA-Seq in basic research for understanding RNA metabolic pathways and gene regulation.

Lexogen has realized a family of kits based on the new genome-wide, non-invasive, quantitative, fast, and reliable SLAMseq (thiol (SH)-Linked Alkylation for the Metabolic Sequencing of RNA) method, developed by Stefan Ameres’ research group at IMBA. Compared to standard RNA-Seq the SLAMseq protocol adds only two extra steps: labeling of the RNA by adding one compound to the culture medium and pre-processing the total RNA before continuing with a standard RNA-Seq protocol. SLAMseq can differentiate between nascent RNA and existing RNA, while standard RNA-Seq measures total steady-state RNA levels only. Hence, SLAMseq allows for parallel quantification of total and newly synthesized RNA levels, without the need for biochemical isolation. Sampling at different time points reveals the complete in vivo and transcriptome-wide kinetics of RNA synthesis and degradation.

Think of what you can do when you know RNA transcription and degradation rates transcriptome-wide...!
lexogen is offline   Reply With Quote

decay, metabloic, nascent, rna-seq, slamseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:56 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO