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Old 03-31-2011, 05:44 PM   #1
whimsy
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Default Can smRNA sequencing primer and genomic DNA sequencing primer mixed in one lane?

Hi, everyone. This is my first post here. I'm new to GAII sequencing. Here is my question:

Can I mix small RNA sequencing primer and genomic DNA sequencing primer in one lane while synthesis of the first-base? Because this lane contains two samples require different sequencing primers.

Thanks.
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Old 04-04-2011, 10:17 AM   #2
volks
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how would you separate the two samples?
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Old 04-05-2011, 05:24 PM   #3
whimsy
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by adding a "barcode" at the 5' end of the library, so that I can separate the two samples after sequencing.
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Old 04-06-2011, 09:30 AM   #4
hoisinjl
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As long as you can tell the samples apart, there is no issue. Illumina v2 CBOTs kits have a cocktail of primers, including the genomic and small RNA primers in the same lane.
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