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Old 07-27-2011, 04:48 AM   #1
yuelics
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Location: Toronto, Canada

Join Date: Apr 2011
Posts: 13
Default Quantification: count reads or count base pairs?

Hi all,

I am new to the field. Here is a silly question:

For quantification of defined genomic regions (let it be genes, windows of a fixed size, etc) based on sequencing data, why do people rarely count base-pairs within the regions but rather count reads.

Could anyone shed some light on the dis/advantages for counting base-pairs/reads?

Thanks in advance
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