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Old 01-07-2012, 04:15 PM   #1
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Location: Stanford

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Default tophat mapping of pair ended library from scriptseq

Hi, I wonder when I do tophat mapping for library generated by epicenture Scriptseq kit, which "library type" option should I use? Should it be" fr-unstranded"? From the protocol description, looks like the reads from primer 1 is sense and the reads from primer 2 is antisense.Thanks!
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Old 05-21-2012, 05:51 AM   #2
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I'm not sure which library type should be used, as well.

Anyone can help?
MarcoMagi is offline   Reply With Quote

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