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Old 01-23-2013, 11:08 PM   #1
Rob2012
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Default Illumina single-ended reads

Hi All,

I would like to learn RNA-seq analysis, but unsure of where to get "Illumina
single-ended reads" to get me started. I have seen the tutorial on wikiRNA-seq but could not find any link to download reads for alignment to a reference. I will be happy if someone can point me in the right direction.

Thanks!


Rob
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Old 01-24-2013, 03:11 AM   #2
GenoMax
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Use either http://sra.dnanexus.com/?result_type...show=&q=rnaseq, NCBI SRA http://www.ncbi.nlm.nih.gov/sra?term=rnaseq or EBI ENA http://www.ebi.ac.uk/ena/data/search?query=rnaseq.

The files will generally be large so have some patience, storage space and time handy.
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Old 01-24-2013, 09:32 AM   #3
kwaraska
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I would like to point out that a majority of our customers doing RNAseq run Paired and runs so they can remove PCR duplicates.

You might be better off trying a PE data set
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Old 02-11-2013, 07:58 PM   #4
Rob2012
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Many thanks to the two of you for your very helpful suggestions.

Rob
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read alignment, rna-seq data analysis, rna-seq mapped reads

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