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Old 02-15-2013, 01:07 PM   #1
Smriti
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Default Target resequencing Vs Amplicon sequencing

Hi there,

I am relatively new to the field of DNA sequencing, so asking a basic question here:

What is the difference between Target Resequencing and Amplicon Sequencing? I guess both are done to study targeted regions of genome and compared against some reference.

Thanks!
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Old 03-05-2013, 02:27 PM   #2
swbarnes2
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Amplicon resequencing is a kind of targeted resequencing

With amplicon sequencing, you do PCR with two primers flanking the region you care about. Presumably, you'd try to multiplex a bunch of them together per sample. Then you make a sequencing library of all those amplicons

The other common way to sequence a particular subset of a genome is to use a capture probe that binds to your sequence of interest, then you sequence that library. Large sets of probes designed to capture exonic sequence are commonly used.
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Old 04-05-2013, 12:09 PM   #3
rastare1a
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Thanks for this question smriti, I'm interested in methods or kits that can be used with capture probes, or perhaps a good review article on the subject.
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