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Old 09-26-2013, 05:41 PM   #1
arkilis
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Location: Australia

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Default NEWBIE question: does the 5' adapter mean forward primer?

As in the title.

3' adapter == reverse primer
5' adapter == foward primer

Is that right? Thanks!
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Old 09-26-2013, 06:13 PM   #2
Kennels
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No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.
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Old 09-26-2013, 08:37 PM   #3
arkilis
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Quote:
Originally Posted by Kennels View Post
No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.
thx~~ it is much clear to me now

Last edited by arkilis; 09-26-2013 at 09:24 PM.
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