SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > SOLiD



Similar Threads
Thread Thread Starter Forum Replies Last Post
454 quality filtering GSCHALLA 454 Pyrosequencing 0 01-30-2012 02:57 PM
Should I do any quality filtering in such a case? GloriaFu Bioinformatics 4 06-10-2011 11:51 AM
454 amplicon quality filtering JackieBadger Bioinformatics 10 03-16-2011 01:28 PM
Filtering on quality Farhat Bioinformatics 4 05-19-2010 06:24 AM
Read quality filtering for long, PE runs kmcarr Illumina/Solexa 0 07-21-2009 07:07 AM

Reply
 
Thread Tools
Old 05-13-2010, 11:42 PM   #1
Farhat
Member
 
Location: Pune, India

Join Date: Apr 2008
Posts: 21
Default Quality filtering

What kind of trimming and/or quality filter do you use before mapping reads? Are there any commonly used filtering criteria? I am using Q>15 and number of bases allowed outside this set at 6, for 32 bp reads (4 bases trimmed from the 3' end). This leaves me with around 15% of the total reads.
__________________
Farhat Habib
Farhat is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:25 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO