Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Making small RNA lib. - do I need DNAse? JonB Sample Prep / Library Generation 4 07-22-2013 12:45 AM
Truseq RNA kit for making DNA library elisadouzi Sample Prep / Library Generation 3 07-17-2013 07:32 AM
CASIM: Variants Making use of array genotype data casim UK - Cambridge 0 04-18-2013 12:32 PM
Does small RNA sequencing generate comparable reads as regular Solexa sequencing? Goodluck Illumina/Solexa 1 08-19-2009 01:12 AM

Thread Tools
Old 01-16-2017, 09:09 PM   #1
Junior Member
Location: india

Join Date: Jan 2017
Posts: 2
Default Making RNA-seq Data comparable


I have rna-seq data from two different tissues collected at different stress conditions. I have performed adapter removal using Cutadapt and I have used TopHat for alignment to the reference genome. What is the ideal method to combine the read-counts from the different datasets ? Should I perform CuffDiff on individual stress-tissue dataset, extract the FPKM files from each of the experiments and merge them using some normalization method ? I need a way to normalize the read-counts across all the stress-tissue datasets because I want to construct co-expression network using RNA-seq data. Any suggestion will be very helpful. Thanks.
sanchari24 is offline   Reply With Quote

rna-seq analysis

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:32 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO