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  • Error in samtools merge; different target sequence name

    I am now making a script with bowtie and samtools, and
    troubled in merging bam files from bowtie.
    1) bowtie with every chromosome ewbt; output is sam file
    2) sam to bam (every chromosome sam file)
    3)* merge all bams
    4) bam sort
    5) bam index

    #!/bin/sh
    date=`date +"%Y%m%d_%H%M"`
    samtools merge ${date}.bam ${date}_bam_chr1.bam, ${date}_bam_chr2.bam,... ,${date}_bam_chrY.bam

    error massage was
    [bam_merge_core] different target sequence name : 'chr1' != 'chr2' in file 20110115_0050_bam_chr2.bam

    In this case, date was 20110115_0050.
    What was wrong??

  • #2
    Hi,

    I had the same problem.

    Check that chromosomes headers are in the same order in the headers of all the bam files.

    Cheers

    Comment

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