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Hi all, I'm preparing to do a new sequencing run on the Ion Torrent PGM. I use the NebNext Fast DNA Fragmentation & Library Prep kit to prepare my library. I, as most, would like to be as cost efficient as possible and wondered if anyone had tried to reduce the amount of reagents used per reaction by half so that I could prep 2 samples as opposed to just one with the same amount of kit components. Thoughts?
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM