Tweet
Hi has anyone used EGS or DSG for fixing haematopoietic cells? Trying to optimise fixation of a cell line using EGS first before Formaldehyde due to the structure of the TF complex. Many protocols suggest EGS of 30-45 minutes, i have found even just 20 minutes to be over kill. The formaldehyde is methanol free single ampules. When fixing with just formaldehyde the sonnication profile on the bioanalyser is ideal yet with EGS then formaldehyde i keep seeing 2 peaks, one around 200bp as expected and another around 3kb. Any suggestions?
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM