Hi all,
I'm looking to amplify specific dinucleotide repeats from low input DNA (~1ng) but I'm having problems with microsatellite contraction/expansion during rounds of PCR. Has anyone with experience in this area have a protocol that can amplify dinucleotide repeat tracts without contracting/expanding the repeat?
Thanks,
Joe
I'm looking to amplify specific dinucleotide repeats from low input DNA (~1ng) but I'm having problems with microsatellite contraction/expansion during rounds of PCR. Has anyone with experience in this area have a protocol that can amplify dinucleotide repeat tracts without contracting/expanding the repeat?
Thanks,
Joe
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