Yes, the method is quite complex, and time consuming as well. I am thinking of just purchasing custom 5´ RNA adapters for simplicity's sake. However, attaching a barcode to the illumina supplied 3´ adapter sounds much cheaper, could you point me in the direction of a protocol?
Also, as I understand it, you are adding the bar code to the adapters that come with the illumina kit? Is this correct?
Thanks so much for you help, I am brand new to multiplexing and nextgen sequencing.
Also, as I understand it, you are adding the bar code to the adapters that come with the illumina kit? Is this correct?
Thanks so much for you help, I am brand new to multiplexing and nextgen sequencing.
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