I am preparing the library for single end RNA-seq following the illumina protocol. However, by agilent analysis for library, the two smear bands occur at 200bp and 500 bp. I do not know why the 500 bp band is visible. Who has the same experience? Anyone's idea for this result?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Originally posted by peromhc View PostWhat stage of the library prep are you at?? Is this the final purified library? What size bands are you selection?
Comment
Latest Articles
Collapse
-
by seqadmin
The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
Channel: Articles
03-08-2024, 10:41 AM -
-
by seqadmin
Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...-
Channel: Articles
02-26-2024, 02:07 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 03-14-2024, 06:13 AM
|
0 responses
32 views
0 likes
|
Last Post
by seqadmin
03-14-2024, 06:13 AM
|
||
Started by seqadmin, 03-08-2024, 08:03 AM
|
0 responses
72 views
0 likes
|
Last Post
by seqadmin
03-08-2024, 08:03 AM
|
||
Started by seqadmin, 03-07-2024, 08:13 AM
|
0 responses
80 views
0 likes
|
Last Post
by seqadmin
03-07-2024, 08:13 AM
|
||
Started by seqadmin, 03-06-2024, 09:51 AM
|
0 responses
68 views
0 likes
|
Last Post
by seqadmin
03-06-2024, 09:51 AM
|
Comment