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Hello, we are trying to quantify the expresion of human EB's vs human ES's RNA levels and splice variants for this we are doing a RNA Seq of both cell lines using 100bp PE. We've never done RNA seq before, how many lanes per sample do you need to get a good coverage so that we can tell if one splice variant is more frequent in one cell line than another???
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I am not yet an expert on this, but based on the amount of sequence being generated by HiSeq 2000 and the size of the transcriptome, I'm guessing one lane per sample. Anyone know for sure?Mendelian Disorder: A blogshare of random useful information for general public consumption. [Blog]
Breakway: A Program to Identify Structural Variations in Genomic Data [Website] [Forum Post]
Projects: U87MG whole genome sequence [Website] [Paper]
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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