Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Illumina Nextra prep without using Illumina reagents

    Hi everyone,
    I was hoping some here could help me, i was wondering if there was anyway to sequencing on a miseq machine without having to use Illumina prep reagents. I know the cartridge that goes into the machine needs to be from illumina but i wanted to see if there is a way to do the prep without having to buy there expensive reagents.

  • #2
    Originally posted by crsimao View Post
    Hi everyone,
    I was hoping some here could help me, i was wondering if there was anyway to sequencing on a miseq machine without having to use Illumina prep reagents. I know the cartridge that goes into the machine needs to be from illumina but i wanted to see if there is a way to do the prep without having to buy there expensive reagents.
    Realistically? No. I don't think so.

    --
    Phillip

    Comment


    • #3
      By 'prep' do you mean library prep? - if so, then yes, there are lots of ways of building libraries that don't require Illumina kits. There are home brew methods and other suppliers (e.g. the NEB next range). If you dig a little deeper online you will find some protocols (e.g we use; http://www.ncbi.nlm.nih.gov/pubmed/21431776) - the only advice I would give is to be certain of your adapter sequences as there are a number of historical tweaks that have been made.

      Comment


      • #4
        Look at this paper for home brew Nextera library prep:http://genome.cshlp.org/content/24/12/2033.full It is only worth if you are going to prepare lots of libraries and have facilities for protein expression and purification.

        Comment


        • #5
          Originally posted by crsimao View Post
          Hi everyone,
          I was hoping some here could help me, i was wondering if there was anyway to sequencing on a miseq machine without having to use Illumina prep reagents. I know the cartridge that goes into the machine needs to be from illumina but i wanted to see if there is a way to do the prep without having to buy there expensive reagents.
          Also, you can visit http://lucigen.com/NextGen-Seq/ to find protocols and kits to make fragment and mate pair libraries for sequencing on Illumina platform.

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM
          • seqadmin
            Techniques and Challenges in Conservation Genomics
            by seqadmin



            The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

            Avian Conservation
            Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
            03-08-2024, 10:41 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Yesterday, 06:37 PM
          0 responses
          7 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, Yesterday, 06:07 PM
          0 responses
          7 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-22-2024, 10:03 AM
          0 responses
          49 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-21-2024, 07:32 AM
          0 responses
          66 views
          0 likes
          Last Post seqadmin  
          Working...
          X